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Cultrex RGF Basement Membrane Extract, Type 2
Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand
Be the first to write a review!
Citations:
(42)
Supplier Page
Supplier Page from
R&D Systems, a Bio-Techne Brand for
Cultrex RGF Basement Membrane Extract, Type 2
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Description
For Robust Organoid Cultures - Reduced Growth Factor Basement Membrane Extract
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Cultrex RGF Basement Membrane Extract, Type 2
Company
R&D Systems, a Bio-Techne Brand
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
3533-005-02
Quantity
5 mL
Brand/Trademark
R&D Systems, a Bio-Techne Brand
Applications
Bioactivity
Format
12 - 18 mg/mL
Species
Mouse
Type
Basement Membrane Extract
Storage
Product is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles.
Storage Buffer:
Dulbecco’s Modified Eagle’s medium without phenol red, with 10 µg/ml gentamicin sulfate.
Sterility Testing:
PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines
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R&D Systems, a Bio-Techne Brand
614 McKinley Place N.E.
Minneapolis
,
Minnesota
55413
United States
Phone
: 1 (800) 343-7475
1 (612) 379-2956
Fax: 1 (612) 656-4400
Company Profile
Website:
http://www.rndsystems.com
Citations (42)
(21)
The aryl hydrocarbon receptor contributes to tissue adaptation of intestinal eosinophils in mice
The Journal of experimental medicine
April 4, 2022
Diny NL, Schonfeldova B, Shapiro M, Winder ML, Varsani-Brown S, Stockinger B.
with 20 μl/96-well of
rat
collagen 1 (#
3447-020-01
;
R&D Systems
), Matrigel (Cultrex RGF BME Type II; #
3533-005-02
;
R&D Systems
), 0.1 mg/ml laminin (#
11243217001
;
Roche
), or 1 mg/ml fibronectin (#
F4759
;
Sigma-Aldrich
)
(22)
Retinoic acid signaling drives differentiation toward the absorptive lineage in colorectal cancer
iScience
December 17, 2021
Roelof A. Wester, Lisa van Voorthuijsen, Hannah K. Neikes, Jelmer J. Dijkstra, Lieke A. Lamers, Siebren Frölich, Maarten van der Sande, Colin Logie, Rik G.H. Lindeboom, Michiel Vermeulen
proteins","Chemicals, peptides, and recombinant proteins" ,, Cultrex RGF BME Type 2 PathClear,
R&D Systems
,Cat#
3533-005-02
Advanced DMEM/F-12
,
Gibco
,Cat#
12634028
Cultrex Organoid Harvesting Solution
,
R&D Systems
,Cat#
3700-100-01
Recovery Cell Culture Freezing Medium
(23)
Generation of mixed murine organoids to model cellular interactions
STAR protocols
December 17, 2021
Krotenberg Garcia A, van Rheenen J, Suijkerbuijk SJE.
proteins" ,, Cultrex PathClear Reduced Growth Factor
Basement Membrane
Extract Type 2,
R&D Systems
,
3533-005-02
Advanced DMEM F/12
,
Thermo Fisher Scientific
,12634-010 HEPES,Thermo Fisher Scientific,15630-056 Penicillin/streptomycin,
Thermo Fisher Scientific
(24)
Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer
Cell stem cell
August 5, 2021
Lõhmussaar K, Oka R, Espejo Valle-Inclan J, Smits MHH, Wardak H, Korving J, Begthel H, Proost N, van de Ven M, Kranenburg OW,
et al
.
(25)
Early invasion of the bladder wall by solitary bacteria protects UPEC from antibiotics and neutrophil swarms in an organoid model
Cell reports
July 20, 2021
Sharma K, Thacker VV, Dhar N, Clapés Cabrer M, Dubois A, Signorino-Gelo F, Mullenders J, Knott GW, Clevers H, McKinney JD.
(26)
Active elimination of intestinal cells drives oncogenic growth in organoids
Cell reports
July 6, 2021
Krotenberg Garcia A, Fumagalli A, Le HQ, Jackstadt R, Lannagan TRM, Sansom OJ, van Rheenen J, Suijkerbuijk SJE.
,
ThermoFisher Scientific
,17504-044 Cultrex PathClear Reduced Growth Factor
Basement Membrane
Extract Type 2,
R&D Systems
,
3533-005-02
N-acetylcysteine,Sigma-Aldrich,A9165 TryplE,Thermo Fisher Scientific,12605-010 Dapi,
Thermo Fisher Scientific
(27)
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
Journal of Visualized Experiments
April 19, 2021
Lauren A. Shechtman, Christina M. Piarowski, Jennifer K. Scott, Erin J. Golden, Dany Gaillard, Linda A. Barlow
"
Collagenase from Clostridium histolyticum
, type I",
Sigma
Life Science,C0130-1G, "Cultrex RGF BME, Type 2, Pathclear",
R&D Systems
,
3533-005-02
,Matrigel "Dispase II (neutral protease, grade II)",Sigma-Aldrich (
Roche
),
4942078001
, Disposable Filters,
Sysmex
,
04-0042-2316
, Dulbecco’s
(28)
Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation
eLife
April 9, 2021
Lamers MM, Mykytyn AZ, Breugem TI, Wang Y, Wu DC, Riesebosch S, van den Doel PB, Schipper D, Bestebroer T, Wu NC,
et al
.
05001
, Other,TryplE,
Thermo Fisher scientific
,Cat#
12605010
, Other,
Basement membrane
extract,
R and D Systems
,Cat#
3533-005-02
, Other,
Transwell
inserts,
Corning
,Cat#
3460
, Other,"Collagen Type I, High concentration Rat tail",
Corning
,Cat#
(29)
Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids
Nature protocols
April 1, 2021
Dekkers JF, van Vliet EJ, Sachs N, Rosenbluth JM, Kopper O, Rebel HG, Wehrens EJ, Piani C, Visvader JE, Verissimo CS,
et al
.
II (Thermo Fisher Scientific, cat. no. 17101–015) Cultrex RGF BME, Type 2 (
R&D Systems
, cat. no.
3533–005-02
); Matrigel (e.g.,
BD Biosciences
, cat. no.
356231
) can be used as an alternative with equal performance
(30)
SARS-CoV-2 entry into human airway organoids is serine protease-mediated and facilitated by the multibasic cleavage site
eLife
January 4, 2021
Mykytyn AZ, Breugem TI, Riesebosch S, Schipper D, van den Doel PB, Rottier RJ, Lamers MM, Haagmans BL.
,
Thermo Fisher Scientific
,Cat#
12605010
, "
Cultrex Basement Membrane Extract, Type 2
",
Basement membrane
extract,
R&D Systems
,Cat#
3533-005-02
, "12 mm
Transwell
with 0.4 µm Pore Polyester
Membrane
Insert, Sterile",
Transwell
inserts,
Corning
,Cat#
(31)
Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations
Frontiers in cell and developmental biology
January 1, 2021
Bogacheva MS, Harjumäki R, Flander E, Taalas A, Bystriakova MA, Yliperttula M, Xiang X, Leung AW, Lou YR.
a non-adhesive 96-well plate (
Corning
,
3474
) or in Cultrex® Reduced Growth Factor BME (
R&D Systems
,
3533-005-02
) in angiogenesis 15-well slides (ibidi, Cat# 81501, uncoated). The
cell-number
-to-medium-volume ratio
(32)
Establishment of human fetal hepatocyte organoids and CRISPR-Cas9-based gene knockin and knockout in organoid cultures from human liver
Nature protocols
January 1, 2021
Hendriks D, Artegiani B, Hu H, Chuva de Sousa Lopes S, Clevers H.
(33)
Establishment of patient-derived cancer organoids for drug-screening applications
Nature protocols
October 1, 2020
Driehuis E, Kretzschmar K, Clevers H.
(34)
Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells
STAR protocols
June 19, 2020
Wörsdörfer P, Rockel A, Alt Y, Kern A, Ergün S.
Biotec,130-103-922 Ascorbic acid (Vit.C),Sigma-Aldrich,A4545
Accutase
,
Sigma-Aldrich
,
A6964
BME,
Biotechne
,
3533-005-02
hESC
Matrigel,
Corning
,
354277
Agarose,Biozym,840006 Hematoxylin,Chroma,50837 NaJO3,Merck,7412159 KAl(SO4)2,
AppliChem
,
A2811
C2H3Cl3O2,
AppliChem
,
A4431
C6H8O7,AppliChem,A1350 Paraformaldehyde,Sigma-Aldrich,P6148 Eosin
(35)
Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer
Cell
April 16, 2020
Ruscetti M, Morris JP 4th, Mezzadra R, Russell J, Leibold J, Romesser PB, Simon J, Kulick A, Ho YJ, Fennell M,
et al
.
well dishes coated with
a 150
μL layer of Cultrex reduced growth factor
basement membrane
extract (
3533-005-02
;
R&D Systems
) and cultured in basal or serum-free conditioned DMEM media for 8 hours. Images of tube
(36)
Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer
Cell stem cell
April 2, 2020
Fumagalli A, Oost KC, Kester L, Morgner J, Bornes L, Bruens L, Spaargaren L, Azkanaz M, Schelfhorst T, Beerling E,
et al
.
cancer
cells from either
organoids
or primary
tumors
were seeded in 20 μl BME (
R&D systems
, cat. no.
3533-005-02
) drops containing 500 cells/drop while Lgr5-
circulating tumor cells
were seeded in 20 μl BME drops
(37)
EPDR1 up-regulation in human colorectal cancer is related to staging and favours cell proliferation and invasiveness
Scientific reports
February 28, 2020
Gimeno-Valiente F, Riffo-Campos ÁL, Ayala G, Tarazona N, Gambardella V, Rodríguez FM, Huerta M, Martínez-Ciarpaglini C, Montón-Bueno J, Roselló S,
et al
.
(38)
Characterization of organoid cultured human breast cancer
Breast cancer research : BCR
December 11, 2019
Goldhammer N, Kim J, Timmermans-Wielenga V, Petersen OW.
(39)
Synergistic cancer immunotherapy combines MVA-CD40L induced innate and adaptive immunity with tumor targeting antibodies
Nature communications
November 6, 2019
Medina-Echeverz J, Hinterberger M, Testori M, Geiger M, Giessel R, Bathke B, Kassub R, Gräbnitz F, Fiore G, Wennier ST,
et al
.
(40)
Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic Therapy
Journal of clinical medicine
November 5, 2019
Driehuis E, Spelier S, Beltrán Hernández I, de Bree R, M Willems S, Clevers H, Oliveira S.
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Be the First to Write a Review!
Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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